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Class 9th Biology Important Long Questions | BISE ALL Boards | New Syllabus 2026

🧬 Important Short Questions for Class 9 Biology (2026) – PCTB Guide

Biology Class 9 (PCTB) is a concept-based subject where short questions play a very important role in exams. Every year, a large portion of the paper comes from definitions, differences, and key concepts.

This guide covers most important short questions for 2026 exams based on the syllabus of Punjab Curriculum and Textbook Board (PCTB).

πŸ‘‰ For more notes and solved papers, visit: https://fifthaacademy.pk/

πŸ“š Chapter 1: Introduction to Biology

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https://images.openai.com/static-rsc-4/KBxYCD7gqDQPWeDj7xv7tayG4Y9F2ajajwazLPIWkmgn6Lw6_inFLEIgFMq4PgJ7RPgx4VQfztwGlLW3uHiCZ8TvDGC9OppcsQ9hpP_NJe2xZvIx59UiD_yevbDGdeMOJrxdtMON2zb3x2Iuth9bdMltuBu0STcnZHPoWjkrgtnG0QAQm42q8ibsIwX6UTRp?purpose=fullsize

Important Short Questions:

  1. Define Biology.
  2. What are the main branches of Biology?
  3. What is meant by levels of organization?
  4. Define tissue, organ, and organ system.
  5. What is bioinformatics?
  6. Define biotechnology.
  7. What is meant by scientific method?
  8. What is observation?
  9. Define hypothesis.
  10. What is theory in science?

πŸ”¬ Chapter 2: Solving a Biological Problem

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Important Short Questions:

  1. What is a biological problem?
  2. Define hypothesis with example.
  3. What is deduction?
  4. Define experimentation.
  5. What is data analysis?
  6. What is conclusion in experiment?
  7. What is control group?
  8. Define variable.

🧬 Chapter 3: Biodiversity

https://images.openai.com/static-rsc-4/UYqTd9mgBifrJLpx-2WSkOIqbcnf5dwsCVl9qkVywQextXhDjOV5rHPH-d7TmkF9YvmjILzZe-8yyreGWKt1KlXqPfv5aHg0z3OeQuBG4oHqYR1PshUase64c8ic80ieRK7o5_dB_o8CYexEHV9jYwb2Yaa1UpPohcbsp6ATY1CnAGqJ3066YOURfb-Pgc3A?purpose=fullsize
https://images.openai.com/static-rsc-4/MSq91Hb5NQvrtHHC66vIjjUQTCaKg7kTqRZHNXCMkgr-IC5TlzQa1GN0guN8UGnpzXK0ASmwp9DIjLPB54zqN4JNCZ-VUqjVr8-I8PB4zhejU_jMCKGAy9Dz37V2Q8NNqoHGVKrR7VHvQeh5jNPrZoiwELmQCJvpjbgOAaKsIIOvVc4-BArOODznocrULL7W?purpose=fullsize
https://images.openai.com/static-rsc-4/Iw16QXE4Ixkepx4pVFgip_gpZEKFwgHIQSFPMywPVESLJiXLpm-hCDn8ZA-_GTIvLRZ6sv1TRqFwjYZtuSf03cujRGcdWRllACwjUqOoHRqYLHgm2mh2tH1topAhkAe0UJn2eUqxUCvpU44qt-Mq2ZerwHzs1Ry2VDdZ2pNsWDvgxRmeFLYRijlMKJDEbxyF?purpose=fullsize

Important Short Questions:

  1. Define biodiversity.
  2. What is taxonomy?
  3. Who is the father of taxonomy?
  4. What is binomial nomenclature?
  5. Name the five kingdoms.
  6. Define species.
  7. What is classification?
  8. Write importance of biodiversity.

🧫 Chapter 4: Cells and Tissues

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Important Short Questions:

  1. Define cell.
  2. What is cell membrane?
  3. Define nucleus.
  4. What is cytoplasm?
  5. Define organelle.
  6. What are tissues?
  7. Name types of animal tissues.
  8. Define plant tissues.
  9. What is diffusion?
  10. What is osmosis?

βš—οΈ Chapter 5: Cell Cycle

https://images.openai.com/static-rsc-4/jM1puPmNcGDmXp8D-W1WsxUTdSbbucgUjVjohy0e5mrEW_XufcC-C7Xrf3PG9rr1H30x8WlUfb1OIIy54JM5ApJTrL_EfQdM-L1AmZT9w2XRM8FGR4qEksp-yVhwbVlZsD5N6Ee-ZbJvqHPPWacaALAgb4sg7HScn5LqPSXBN_FzOuTsKlJmTzBBLnmD7RQt?purpose=fullsize
https://images.openai.com/static-rsc-4/AcYKPUryf0hw1CBAelqJMtOTAyFEGz9uLggv0Gb6hvhyHh-TWmjHpax7qkbb3vbVuSR1PqlpQZbFGjZxpY-bk0YNbvoLZLTq8fCuZDKcOLb0BfBOaNvyP0iNJJsOkZD1cbBkwiSt3ObQveGtp8v8lvEINbkS-FLOxWKyGW2Jvt_mKOgO7cYMluzZ6UCSkuYB?purpose=fullsize

Important Short Questions:

  1. Define cell cycle.
  2. What is mitosis?
  3. Name phases of mitosis.
  4. What happens in prophase?
  5. What is cytokinesis?
  6. Why is mitosis important?

πŸ§ͺ Chapter 6: Enzymes

https://images.openai.com/static-rsc-4/Ygs709BUBUD6Av25S6k6UWpbVTAdbLNYSyk3cdkxZxVwHWaUwipO8hyfXTAfAxt8a9_oC3nt9tY3CHf6ee2RpFN1as2GY0d8Sk6g99IzgbKTPupGaIA6yYYyZ1CDWg7mKfeBORNfBz37Hp1p-ep7vnxhYZTpRRobsoQa-CzpQ5FqeOPNc1SlAJm2jcFG0taH?purpose=fullsize
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https://images.openai.com/static-rsc-4/_6sn4e-9jj8lYVRM6z_40HBw52hTorfOPAetXIm8cIV2qoaZ-Gl4tM43UOZcM4i8XOmGyJsmJ0cpcgKlWxsZb5HJeldLgiCL-i_kmC79yaPVg4JLGIdKZN1VmehRZ5re4u9q7-orhYGTrEw6FDAEOx9AVSgr4AVAFjVB4jlQcsLcbJL6G4xsksTmHZNW1FaA?purpose=fullsize

Important Short Questions:

  1. Define enzyme.
  2. What is substrate?
  3. What is active site?
  4. Define catalyst.
  5. What factors affect enzyme activity?
  6. What is optimum temperature?
  7. What is optimum pH?

🌱 Chapter 7: Bioenergetics

https://images.openai.com/static-rsc-4/SO5R3XzaYk7njBNEaGma1RJVsR3e7oKvka69TDARM9hgh4lKFb0JY6FFyaa-IRJLY1f3G4q1iuc0ctognY2SD9io3BVyq8cIr60sgApHnGhxowsk-xw3oLXXeLG9hW2fb04jZN3A_veBx1dhBKNvRpeNCC_BweNAclIWhUpcNMi24lpKRGNUgzU6KLDumP5h?purpose=fullsize
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https://images.openai.com/static-rsc-4/AqXgClZN91ztMH_eNg7TWAHQDGZiQXKxCNcCqxgS1YU4mjRVphn4-CH3rf_xFw1RHgJlw-G-kNMHqWEZlsBBtVKxaZq0yrAa-C2OGyrcGsdJgKRFZiPXDg6SpA90y8A1R8wfs7NxtFgYGIW5Bik2JDUTHQNV3NDsYChYKtSfPlFxpv_2xOgGMajVAAlbS-aA?purpose=fullsize

Important Short Questions:

  1. Define photosynthesis.
  2. Write equation of photosynthesis.
  3. What is respiration?
  4. Define aerobic respiration.
  5. Define anaerobic respiration.
  6. What is ATP?

πŸ₯— Chapter 8: Nutrition

https://images.openai.com/static-rsc-4/6b7sFquruGg-3pQU3o3VP0Gaz8Z8e2ej3ho2XgEB8CCx-51t_idpcxXg6QBBYjH8rtlEly7eQu0hHS6kivWZmKjSsHid6CRvPLgV3IucmLTquTyZH6TvxBfCgTQPaGoeHCnIVZX7DyOjqZl4iNhbh-U70AoqM7nUOSuujYhqApfUjGGsUvpi6T37mPBDYtep?purpose=fullsize
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6

Important Short Questions:

  1. Define nutrition.
  2. What are nutrients?
  3. Define balanced diet.
  4. What is digestion?
  5. What is ingestion?
  6. What is absorption?

πŸš€ Exam Preparation Tips (2026)

  • Focus on definitions & diagrams
  • Revise daily short questions
  • Practice past papers
  • Make your own notes

πŸ‘‰ Get more study material here: https://fifthaacademy.pk/amhb-study-house/

Class 9 Biology Important short question answers

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